What are the main components of the chemically defined synthetic medium for Staphylococcus aureus as described in Table 3.6A?
The chemically defined synthetic medium for Staphylococcus aureus includes specific amino acids, vitamins, and salts. It contains cystine, histidine, leucine, lysine, tryptophan, arginine, glycine, isoleucine, proline, serine, aspartic acid, glutamic acid, phenylalanine, methionine, tyrosine, threonine, and valine. Additional components are nicotinamide, thiamine, pyridoxine, biotin, magnesium sulfate, dipotassium hydrogen phosphate, sodium chloride, and iron chloride.
The chemically defined synthetic medium for Staphylococcus aureus is composed of precise amounts of amino acids, vitamins, and salts. Specifically, it includes 0.25 grams each of cystine, histidine, leucine, lysine, and tryptophan; 0.5 grams each of arginine, glycine, isoleucine, proline, and serine; and 0.12 grams each of aspartic acid, glutamic acid, phenylalanine, methionine, tyrosine, threonine, and valine. The medium also contains 0.005 moles each of nicotinamide, thiamine, and pyridoxine, along with 0.5 micrograms of biotin. For salts, it includes 1.25 grams each of magnesium sulfate, dipotassium hydrogen phosphate, and sodium chloride, as well as 0.125 grams of iron chloride. These ingredients are dissolved in 1,000 milliliters of distilled water and buffered to a final pH of 7.0.
Key points
- The medium includes specific amino acids like cystine, histidine, leucine, lysine, and tryptophan.
- It contains vitamins such as nicotinamide, thiamine, pyridoxine, and biotin.
- Salts included are magnesium sulfate, dipotassium hydrogen phosphate, sodium chloride, and iron chloride.
- The medium is dissolved in 1,000 milliliters of distilled water and buffered to pH 7.0.
Talaro's Foundations in Microbiology
Kathleen Park Talaro
EIGHTH EDITION · McGraw-Hill, a business unit of The McGraw-Hill Companies, Inc.